Biomolecular NMR & Protein Engineering
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equal contribution. * corresponding author, Click each ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() deficiency lead to structural destabilization and impaired binding of U12 snRNA. RNA 24, 396-409. doi: 10.1261/rna.062844.117. ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Use of biosynthetic fractional 13C-labeling for backbone NMR assignment of proteins J. Biomol. NMR, 37, 187-193 ![]() Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS lett. 580, 1853-1858. Suppl. ![]() Intein-based biosynthetic incorporation of unlabeld protein tags into isotopically labeled proteins for NMR studies. Nature Biotechnology. 23, 736-740. Suppl. [News 1, 2] ![]() NMR solution structure of the monomeric form of the bacteriophage lambda capsid stabilizing protein gpD. J. Biomol. NMR. 31, 351-6 ![]() NMR structures of salt-refolded forms of the 434-repressor DNA-binding domain in 6 M urea. Biochemistry 43,13937-43 ![]() NMR structure of a variant 434 repressor DNA-binding domain devoid of hydroxyl groups. J. Biomol. NMR. 29, 395-8. ![]() Channel forming membrane permeabilization by an antibacterial protein: Sapecin; determination of membrane-buried and oligomerization surfaces by NMR. J. Biol. Chem. 279, 4981-4987 ![]() Assignments of 1H and 15N resonances of the bacteriophage lambda capsid stabilizing protein gpD. J. Biomol. NMR. 28, 89-90 1993-2003![]() Crystal structure of cyclized green fluorescent protein. Acta Crystallographica. D 58, 1400-6 ![]() Modern methods for isotope labelling of proteins in BioNMR Techniques in drug research (Wiley-VCH). ![]() Effects of backbone cyclization on protein stability and folding. Nova Acta Leopoldina suppl. 16 135-136. ![]() Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus. J. Biol. Chem. 276, 16548-54. ![]() Influence of the pKa value of the buried, active-site cysteine on the redox properties of thioredoxin-like oxidoreductases FEBS lett. 477, 21-26 ![]() Circular β-lactamase: stability enhancement by cyclizing the backbone. FEBS lett. 459, 166-172. ![]() Conformational Changes of the BS2 Operator DNA upon Complex Formation with the Antennapedia Homeodomain Studied by NMR with 13C/15N-labeled DNA. J. Mol. Biol. 292, 609-617. ![]() Expression, stabilities, and NMR studies of hydration of recombinant variants of the DNA binding domain of phage 434 repressor. Diss. ETH Nr. 12684 ![]() NMR with 13C, 15N-doubly-labeled DNA: the Antennapedia homeodomain complex with a 14-mer DNA duplex. J. Biomol. NMR 12, 25-37 ![]() Solvent magnetization artifacts in high-field NMR studies of macromolecular hydration. J. Magn. Reson. 130, 262-271 ![]() Measurement of 3Jc2'p scalar couplings in a 17kDa protein complex with 15N/13C labelled DNA distinguishes the BI and BII phosphate conformations of the DNA. J. Am. Chem. Soc. 119, 9901-9902. ![]() Arginine side chain assignments in uniformly 15N-labeled proteins using the novel 2D HE(NE)HGHH experiment. J. Biomol. NMR 10, 193-197. ![]() Anisotropic molecular rotational diffusion in 15N spin relaxation studies of protein mobility. Biochemistry 36, 7305-7312. ![]() The 2D NMR experiments H(C)CO2 and HCCO2 for assignment and pH-titration of carboxylate groups in uniformly 15N/13C labelled proteins. J. Magn. Reson. 124, 274-278. ![]() Three-dimensional solution structure of the calcium channel antagonist omega-agatoxin IVA: consensus molecular folding of calcium channel blockers. J. Mol. Biol. 250, 659-671. ![]() Synthesis and characterization of sapecin and sapecin B FEBS Lett. 342, 189-92. ![]() Solution conformation of an antibacterial peptide, sarcotoxin IA, as determined by 1H-NMR. Eur. J. Biochem. 217, 639-44. Presentation. (I:invited) H.Iwai (2017) Segmental isotopic labeling of proteins. Advanced Isotopic Labelling Methods for Integrated Structural Biology, March. 10–13, 2017, Grenoble, France.. H.Iwai (2016) Protein ligation for protein NMR and beyond. Swedish NMR Centre 25 year anniversary symposium. Oct. 13. 2016. Göthenburg, Sweden. H.Iwai (2016) Protein Engineering of HINT domains for Protein Ligation. EMBO conference: The biochemistry and chemistry of biocatalysis: From understanding to design. 12/06/2016 -15/06/2016 Oulu, Finland H.Iwai (2015) Protein Splicing and related phenomena: Tools for Protein NMR. NMR symposium, 10th December, 2015. Trondheim, Norway. H.Iwai (2014 ) Inteins. Symposium Biomolecular NMR Spectroscopy. 10-11 Nov 2014, Biozentrum, Basel. Switzerland. H.Iwai (2013) Structure-guided protein design towards segmental isotopic labeling of globular, multi-domain, and membrane proteins. 3rd BioNMR Annual User Meeting: NMR and protein dynamics in structural biology. Hotel Benczur, Budapest, Hungary. June. 10–13, 2013. H.Iwai (2012) Backbone cyclization by novel split protein-splicing domains: lessons from Kalata B1. Heron Island, Australia. Oct. 15–17, 2012. (I) H.Iwai (2012) Recent advancement in segmental isotopic labeling of multi-domain proteins. the XXVth International Conference on Magnetic Resonance in Biological System. Cité center de congrés, Lyon, France. Aug. 19–24, 2012. H.Iwai (2011) Stable Isotopic Labeling of Proteins for Biomolecular NMR in Post-structural Genomics Era. The international ISNMR2011, the 50th Memorial Annual Meeting of the NMR Society of Japan. . Yokohama, Japan. Nov. 15–18, 2011. (I) H.Iwai (2010) Protein and peptide ligation by split inteins. 5th International Peptide Symposium. Kyoto International Conference Center. Kyoto, Japan. December 4–9, 2010. H.Iwai (2010) Protein splicing in cis and trans: a versatile tool for structural biology and post-translational modulation of protein functions. 35th FEBS Congress, Götenborg, Sweden. June 26-July 1, 2010 (I: FEBS lett. young group leader Lecture) H.Iwai (2009) Protein trans-splicing as an enabling tool in structural biology. The VIII European Symposium of the Protein Society, Kongresshaus, Zürich, Switzerland, June 24-25, 2009 (I) H.Iwai (2008) Segmental isotope-labeling using in vivo and in vitro protein trans-splicing: How to make it work. NMR-Life Workshop BNMRZ "Protein expression and isotope labeling for structural biology", München, Germany, July 24-25, 2008 (I) H.Iwai (2006) Intein-based protein engineering. The 89th CSC conference, Halifax, NS, Canada, 27-31. May, 2006 (I) H.Iwai (2006) Intein-based protein engineering for NMR studies. The 27th Danish NMR Discussion Meeting, Rebuild, Denmark. 9-10. Jan, 2006 (I) Iwai, H. (2004) Exploring protein splicing, Biochemistry seminar, University of Saskatchewan, 29th Sep 2004, Saskatoon, Canada Iwai, H (2003) Application of protein splicing domains. Department of Pharmaceutical Science,Toyama Medical and Pharmaceutical University, 29th September 2003, Toyama, Japan (I) Iwai, H (2002) Protein NMR Spectroscopy Combined with Protein Engineering Approaches'”, Department of chemistry, University of Saskatchewan, 31st October 2002, Saskatoon, Canada (I) Iwai, H. (2002) Protein NMR spectroscopy combined with protein engineering approaches. Nestle Research Institute, Oct 24 2002, Lausanne Switzerland (I) Iwai, H. (2002) Intein-based protein engineering for protein NMR spectroscopy. Max-Plank-Institute, 24th September,2002, Göttingen, Germany Iwai, H. (2002) Protein NMR spectroscopy combined with protein engineering approaches. Salk Institute, March 1,2002,San Diego, USA Iwai, H. (2002) Protein NMR spectroscopy combined with protein engineering approaches. Universitaet Leipzig, 28 January, 2002, Leipzig, Germany Iwai, H., and Plückthun, A. (2000) Effects of backbone cyclization on protein stability and folding. Leopoldina-Symposium "Structure, Self-organization and Stability of Proteins: Experiments and Models" 21. - 23. September, Wittenberg, Germany Iwai, H (2000) NMR Spectroscopy and Protein Engineering: from Hydration to Cyclic Protein. Novartis Pharma, June 16, 2000, Basel, Switzerland Poster J. S. Oeemig, A. S., Aranko, and H. Iwai (2010) DnaE Intein Structure from Nostoc punctiforme and Design of New Split Inteins. Euromar 2010, 17th ISMAR Conference, Florence, Italy, July 4-9, 2010. J. S. Oeemig and H. Iwai (2009) NMR Solution Structure of DnaE Intein from Nostoc punctiforme. June 14-18. The VIII European Symposium of the Protein Society, Kongresshaus, Zürich, Switzerland A. E. L. Busche, C. Hein, A. Koglin, F. Löhr, C. T. Walsh, H. Iwai, and V. Doetsch (2009) Segmental isotopic labeling as a tool to solve the structure of three consecutive ACP domains in the Curacin A synthetase. June 14-18. The VIII European Symposium of the Protein Society, Kongresshaus, Zürich, Switzerland. A.S. Aranko and H. Iwai (2009) How to make protein ligation by protein trans-splicing work , June 14-18. The VIII European Symposium of the Protein Society, Kongresshaus, Zürich, Switzerland. E. Buchinger, F. Aachmann, H. Iwai, R. Wimmer (2009) Segmental labelled AlgE4- an alginate epimerase. The VIII European Symposium of the Protein Society, Kongresshaus, Zürich, June 14-18, Switzerland. H. Iwai, A. S. Aranko J. Djupsjöbacka (2008) Protein ligation using protein trans-splicing. 30th European Peptide Symposium. 31 Aug.-5. Sept. 2008, Helsinki, Finland. J. Djupsjöbacka, M. Muona, A. S. Aranko. and H. Iwai (2008) Protein ligation using protein-trans splicing: toward segmental isotope-labelling of multi-domain proteins for NMR studies. INPEC meeting 2008, June 12 - 15, Naantali, Finland (the best poster award) A. S. Aranko and H. Iwai (2008) Site-specific labelling of proteins using an engineered intein (2008) INPEC meeting 2008, June 12 - 15, Naantali, Finland. K. Pääkkonen, J. Fiaux, and H. Iwai,(2007) ISMAR2007 Optimization of stable-isotope labeling for backbone resonance assignment of proteins, Kenting, Taiwan. Pääkkonen, K. Iwai, H, (2007) Protein backbone resonance assignment from fractional 13C-labeling data. EUROMAR 2007, 1-6, July. Tarragona , Spain. Iwai, H, Mahlakõiv, T., Karjalainen, K. Knuuti, J. (2006) In vivo segmental isotope labeling using trans-splicing. XXIInd ICMRBS 2006, 20-25. Aug. Göttingen, Germany. Iwai, H, Züger, S (2005) New approach to improve protein stability and solubility for NMR studies. VIth European Symposium of the Protein Society,30 Apr-4. May, Barcelona, Spain. Züger, S., Iwai, H. (2005) New approach to improve protein stability and solubility for NMR studies. Keystone Symposia, Frontiers of NMR in Molecular Biology IX. 29. Jan-04 Feb., Banff, Alberta, Canada. Iwai, H., Lingel, A., Taguchi, H. and Plückthun, A. (2001) Circular proteins: stability enhancemen by backbone cyclization. Spetsai Summer School 2001, Protein Biology: From Synthesis to Function and Disease. 4-14 September, Island of Spetses, Greece Iwai, H., Zerbe, O. and Plückthun, A. (2000) Effects of the Backbone Cyclization on Protein Stability and Folding as Characterized by NMR spectroscopy. XIX International Conference on Magnetic Resonance in Biological Systems. August 20-25, 2000, Convention Center - Florence, Italy. Iwai, H., and Plückthun, A. (1999) Circular protein: a strategy for stabilizing proteins. The third protein society meeting, 19-22, September, Garmish-Partenkirchen, Germany. Iwai, H., Sobol, A., Wider, G. and Wüthrich, K. (1998) The complete surface hydration shell of a protein by NMR spectroscopy. XVIII International Conference on Magnetic Resonance in Biological Systems. August 23-28, 1998, Tokyo Metropolitan University, Hachioji , Tokyo, Japan. Last modified on
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